EGS crosslinker, Ethylene glycolbis(succinimidylsuccinate), is a water insoluble homobifunctional crosslinker that must be dissolved in DMSO or DMF before addition to an aqueous reaction. EGS crosslinking takes place through amine-reactive NHS esters at both ends of a 16.1 angstrom spacer arm. The 12-atom spacer arm of the EGS crosslinking reagent is cleavable. EGS crosslinker"s spacer arm is cleaved by hydroxylamine at pH 8.5 for 3-6 hours at 37°C. The EGS protein crosslinker possesses amino reactive NHS esters on both ends of the molecule and can be used to label intracellular proteins, since the molecule is cell membrane permeable. EGS crosslinker is often used in ChIP assays to determine DNA-protein binding interactions. The directly watersoluble analog is of EGS is Sulfo-EGS.
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Molecular Weight: 456.36Spacer Length: 16.1 ÅCAS Number: 70539-42-3Alternative Names: Ethylene glycol bis (succinimidylsuccinate); Ethylene Glycol-Bis (Succinic Acid N-Hydroxysuccinimide Ester)Special bulk pricing is available on bulk quantities of EGS crosslinker for your large volume manufacturing application.ProteoChem是一家生物技术和化学试剂制造公司，专门从事蛋白质生物化学和有机合成。ProteoChem生产用于NMR，质谱，蛋白质交联剂和用于蛋白质检测和蛋白质修饰的新型蛋白质组学试剂的分析标准品，这些试剂用于质谱，药物开发，诊断和蛋白质生物学中。
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