NHS-SS-Biotin, Succinimidyl-2-(biotinamido)-ethyl-1,3"-dithiopropionate, is an amine-reactive biotinylation reagent that is cleavable when the disulfide (-S-S-) bond is reduced.NHS-SS-Biotin is cell membrane permeable and has a long chain separating the biotin group from the amino-reactive NHS-ester (N-Hydroxysuccinimide ester).The resulting biotinylated protein can then be detected when used in conjunction with streptavidin.It is possible to cleave the biotin group from the crosslinked target protein under reducing conditions, such as in the presence of beta-mercaptoethanol (BME), TCEP-HCl or DTT. This technique is often used to purify an antibody or other protein from a biotin specific affinity resin like Immobilized Streptavidin without exposing your protein of interest to denaturing conditions.
Click here for: NHS-SS-Biotin Protocol& Product Information Sheet
Molecular Weight: 504.65Spacer Length: 24.3 ÅCAS Number: 142439-92-7Alternate Names: Biotin disulfide N-Hydroxysuccinimide ester; NHS SS BiotinSpecial bulk pricing is available on bulk quantities of NHS-SS-Biotin for your large volume manufacturing application.ProteoChem是一家生物技术和化学试剂制造公司，专门从事蛋白质生物化学和有机合成。ProteoChem生产用于NMR，质谱，蛋白质交联剂和用于蛋白质检测和蛋白质修饰的新型蛋白质组学试剂的分析标准品，这些试剂用于质谱，药物开发，诊断和蛋白质生物学中。
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