Iodoacetyl-LC-Biotin (N-Iodoacetyl-N"-Biotinylhexylenediamine or N-Biotinyl-N"-(iodoacetyl)-1,6-hexanediamine) reacts with sulfhydryl groups to form stable thioether linkages.Iodoacetyl-LC-Biotin has an extended spacer arm that reduces steric hindrance.Iodoacetyl-LC-Biotin must be dissolved in an organic solvent, such as DMSO or DMF, then added to an aqueous biotinylation reaction. Iodoacetyl-LC-Biotin coupling reactions are most efficient when reacted at at pH 7.5 - 8.5 in reduced light. The resulting biotinylated protein can then be detected when used in conjunction with streptavidin or Immobilized Streptavidin.
Spacer Length: 27.1 ÅMolecular Weight: 510.43CAS Number: 93285-75-7Alternative Names: N-Biotinyl-N"-(iodoacetyl)-1,6-hexanediamine; N-iodoacetyl-N-biotinylhexylenediamine (IAB); Iaa-BiotinSpecial bulk pricing is available on bulk quantities of Iodoacetyl-LC-Biotin reagent for your large volume manufacturing application.ProteoChem是一家生物技术和化学试剂制造公司,专门从事蛋白质生物化学和有机合成。ProteoChem生产用于NMR,质谱,蛋白质交联剂和用于蛋白质检测和蛋白质修饰的新型蛋白质组学试剂的分析标准品,这些试剂用于质谱,药物开发,诊断和蛋白质生物学中。
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