LC-SPDPcrosslinker,Succinimidyl6-(3-[2-pyridyldithio]-propionamido)hexanoate,isacleavable,waterinsoluble,aminoandthiol(sulfhydryl)reactiveheterobifunctionalproteincrosslinker.The"longchain"LC-SPDPcrosslinkingreagenthasa15.7angstromspacerarm,comparedtotheshorter6.8angstromspacerarmversionofSPDPcrosslinker.Often,duetosterichindrances,itisdesirabletoempiricallytestwhichofthetwoLC-SPDPcrosslinkerversionswillworkbestinyourspecificcrosslinkingapplication.
LC-SPDPcrosslinker"sreactivemoitesconsistofanN-hydroxysuccinimide(NHS)esterthatreactswithprimaryamines,asfoundinlysinesidechains.WhileontheotherendoftheLC-SPDPcrosslinkermoleculepyridinyldisulfidereactswithsulhydrylstoyieldareduc
IBLedisulfidebondwhichcanbereducedwithTCEP-HClorDTTtoreversethecrosslinkedproteins.
MolecularWeight:425.5
SpacerLength:15.7Å
SpecialbulkpricingisavailableonbulkquantitiesofLC-SPDPcrosslinkingreagentforyourlargevolumemanufacturingapplication.