DTSSPcrosslinker(3,3´-Dithiobis[sulfosuccinimidylpropionate])isahomobifunctionalproteincrosslinkerthatiscellmembraneimpermeable.DTSSPcrosslinkingreagenthasprimaryamine-reactiveN-hydroxysulfosuccinimide(Sulfo-NHS)estersatbothendsofacleavable,8-atom(12.0angstrom)spacerarm.DTSSPcontainsareduc
IBLedisulfide(thiol)bondinthespacerarmthatmakesitagoodchoiceforvariousapplications.ThedisulfidebondcanbereducedwithTCEP-HClorDTTtoreversethecrosslinkedproteins.
DTSSPcrosslinkingreagentisidealforaqueousconjugationreactions.DTSSPcrosslinkercanbeusedtocrosslinkextracellularandmembraneproteins.Ifintracellularcrosslinkingisdesired,thenDSP,thewaterinsolubleanalogofDTSSPcanbeusedforyourresearch.