DTMECrosslinker(dithiobismaleimidoethane;Dithio-bis-maleimidoethane)isahomobifunctionalandcleavablecrosslinkerthatiscellmembranepermeable.DTMECrosslinkerhassulfhydryl-reactivemaleimidesatbothendsofacleavable,11-atom(13.3angstrom)spacerarm,andDTMEcrosslinksoccurviathesemaleimides.DTMEcrosslinkingreagentcontainsareduc
IBLedisulfide(thiol)bondinthespacerarmthatmakeitagoodchoiceforvariousapplications.ThedisulfidebondmaybereducedwithTCEP-HClorDTTtoreversethecrosslinkedproteins.
DTMECrosslinkermustbedissolvedinanorganicsolvent,suchasDMSOorDMF,thenaddedtoanaqueouscrosslinkingreaction.DTMEcrosslinkingreagentcanbeusedtocrosslinkintracellularproteins.