EDC-HClzero-lengthcrosslinker(EDAC-HCl,EDCorEDAC;EDCI;1-Ethyl-3-[3-dimethylaminopropyl]carbodiimidehydrochloride)from
ProteoChemisultrapure,andisusedtoconjugatecarboxylfunctionalgroupstoprimaryaminesasfoundinpeptidesandproteins.Itisidealforcovalentlybindingproteinsorpeptidestocarboxylcontainingbeads,resins,orothernanoparticals.Theconvenient10mgpackagingisgreatforusingProteoChem"sEDC-HClin
Luminexbeadconjugationreactions.EDC-HCliswatersoluble,thusthereactioncantakeplaceatphysiologicalconditions;althoughthebestresultstakeplaceatpHof5.5orlessbufferedinMES.Sulfo-NHSorNHSisusedasacatalystforEDC-HClcouplingandgreatlyincreasestheefficiencyofthereaction.