ProteoChem"sultrapureEDC-HCl(EDAC-HCl)zero-lengthcrosslinker(1-Ethyl-3-[3-dimethylaminopropyl]carbodiimidehydrochloride)isusedtoconjugatecarboxylfunctionalgroupstoprimaryaminesasfoundinpeptidesandproteins.Itisidealforcovalentlybindingproteinsorpeptidestocarboxylcontainingbeads,resins,orothernanoparticals.EDC-HCliswatersoluble,thusthereactioncantakeplaceatphysiologicalconditions;althoughthebestresultstakeplaceatpHof5.5orlessbufferedinMES.Sulfo-NHSorNHSisusedasacatalystforEDC-HClcouplingandgreatlyincreasestheefficiencyofthereaction.