DSP-d8deuteratedproteincrosslinker,Dithiobis(succinimidyl2,2,3,3,6,6,7,7-propionate-d8),isacellmembranepermeable,homobifunctional,proteincrosslinkercontaining8deuteriumatomstoprovidean8Daltonshiftbymassspectrometrycomparedtonon-dueteratedDSP(DSP-d
0).DSP-d8deuteratedcrosslinkerhasamine-reactiveNHSestersatbothendsofacleavable,8-atom(12.0angstrom)spacerarm.DSP-d8deuteratedcrosslinkercontainsareduc
IBLedisulfidebond(SS-bond)inthespacerarmthatmakesitagoodchoicefornumerousapplicationswherereversiblecrosslinkingisdesired.
UsetheheavyDSP-d8deuteratedproteincrosslinkingreagentincombinationwiththelight,non-deuteratedanalog(DSP-d
0)formassspecstudies.
DSP-d8deuteratedcrosslinkermustbedissolvedinanorganicsolvent,suchasDMSOorDMF,andthenmaybeaddedtoanaqueouscrosslinkingreaction.BecauseDSP-d8crosslinkeriscellmembranepermeableitcanbeusedtocrosslinkintracellularproteins.
MolecularWeight:412.46
SpacerLength:12.0Å
Alternativenames:d8-DSP;DSP-d
8;D8-DSP;Dithiobis(succinimidylpropionate-d8);DTSP-d8
References:
Tan,L.,Turecek,F.,Francisco,J.S.,&Xia,Y.(2014).ProbingtheR
ADIcalandBaseDualPropertiesofPeptideSulfinylRadicalsviaMassSpectrometry.TheJournalofPhysicalChemistryA,118(50),11828-11835.
Rotunno,M.S.,Auclair,J.R.,Maniatis,S.,Shaffer,S.A.,Agar,J.,&Bosco,D.A.(2014).Identificationofamisfoldedregioninsuperoxidedismutase1thatisexposedinamyotrophiclateralsclerosis.Journalof
BIOLOGicalChemistry,289(41),28527-28538.
SpecialbulkpricingisavailableforbulkquantitiesofDSP-d8deuteratedcrosslinkerforyourlargevolumemanufacturingapplication.