Sulfo-NHS-Biotin(Sulfosuccinimidylbiotin)isawatersolublebiotinylationreagentusedinBiotin-Streptavidinbindingreactions.Itisaminereactiveandhasaspacerarmlengthof13.5Å.TheSulfo-NHS-BiotinbiotinylationreagentisidealforbiotinylatingantibodyorproteinthatcanthenbeusedforproteindetectionorimmobilizationwhenusedinconjunctionwithstreptavidinandImmobilizedStreptavidin.Sulfo-NHS-BiotinisparticularlyusefulfortargetingcellsurfaceproteinsinStreptavidin-Biotinassays.ThewaterinsolubleversionofSulfo-NHS-BiotinisNHS-Biotin.
Sulfo-NHS-Biotinandthe"longchain"Sulfo-NHS-LC-Biotinbothhavethesamechemicalfunctionality,butthelatterwillprovidealongerspacerarmmorecapableofovercomingsterichindrancesthatmaybepresentbetweenthelabeledprotein(s)andstreptavidin(
ProteoChemproduct#e7105-5mg).Often,thebestresultsaredeterminedbyempiricallytestingeachreagentforyourspecificapplication.