Sulfo-NHS-LC-Biotin,Sulfosuccinimidyl6-(biotinamido)Hexanoate,isalongchain,watersolublebiotinylationreagentthatisreactivetowardprimaryaminegroups.SimilartoSulfo-NHS-Biotin,Sulfo-NHS-LC-Biotinisdesignedtotargetcellsurfaceproteins.ThesodiumsulfonategroupslendwatersolubilitytoSulfo-NHS-LC-Biotinandpreventitfromcrossingthelipidbi-layerofthecellmembrane;whereasNHS-LC-Biotincanbiotinylateintracellularproteins.TheresultingbiotinylatedproteincanthenbedetectedwhenusedinconjunctionwithstreptavidinandImmobilizedStreptavidin.
The"longchain"Sulfo-NHS-LC-BiotinandSulfo-NHS-Biotinbothhavethesamechemicalfunctionality.However,Sulfo-NHS-LC-Biotinwillprovidealongerspacerarmthatisdesignedtoovercomeanysterichindrancesthatmaybepresentbetweenthelabeledprotein(s)andstreptavidin(
ProteoChemproduct#e7105-5mg).Often,thebestresultsaredeterminedbyempiricallytestingeachreagentforyourspecificapplication.