NHS-SS-Biotin,Succinimidyl-2-(biotinamido)-ethyl-1,3"-dithiopropionate,isanamine-reactivebiotinylationreagentthatiscleavablewhenthedisulfide(-S-S-)bondisreduced.NHS-SS-Biotiniscellmembranepermeableandhasalongchainseparatingthebiotingroupfromtheamino-reactiveNHS-ester(N-Hydroxysuccinimideester).Theresultingbiotinylatedproteincanthenbedetectedwhenusedinconjunctionwithstreptavidin.
Itisposs
IBLetocleavethebiotingroupfromthecrosslinkedtargetproteinunderreducingconditions,suchasinthepresenceofbeta-mercaptoethanol(BME),TCEP-HClorDTT.ThistechniqueisoftenusedtopurifyanantibodyorotherproteinfromabiotinspecificaffinityresinlikeImmobilizedStreptavidinwithoutexposingyourproteinofinteresttodenaturingconditions.