Carbohydrate-ReactiveResin(hydrazide-agarose,HYD-Agarose)isacouplingresinisusedforimmobilizingglyconesandotherglycatedmolecules,includingglycoproteinsandantibodiesthroughtheircarbohydrates(compareto
ThermoScientificCarb
Olinkor
PierceCarbolink*).Carbohydrate-ReactiveResinissuppliedasa50%slurry(15mLresin=30mLslurry).
Carbohydrate-ReactiveAgaroseResinisachemicallymodifiedagarosegelwithahydrazidegroupimmobilizedon6%crosslinkedagarose.Thebeadsizeis45to165um.Itisidealforforcouplingsmallligandscontainingreactiveformylgroupsortoimmobilizeproteinsthatcontainoxidizablecarbohydrates.Forglycoproteins,thecouplingreactiontakesplacebyoxidizingthecarbohydrategrouponyourglycoproteinwithSodiummeta-Periodateandcombiningtheoxidizedglycoproteinwiththeresin.Thespacerarminthisresinenablesincreasedcouplingefficiencybyreducingsterics.