ImmobilizedPepsinresin(PepsinAgarose)iscommonlyusedtocreateF(ab)2fragmentsfromantibodies.Byimmobilizingpepsinautolysisandproteasecontaminationisessentiallyeliminated.ImmobilizedPepsingelhydrolyzesproteinnon-specificallyatpH1.0to3.0,withanoptimumactivityatpH1.0.Ourhighlyactivepepsiniscovalentlyboundtocrosslinked6%beadedagaroseresinanditsspecificactivityis>6,100units/mlresin(oneunitisdefinedasreleasing0.001A280asTCAhydrolyzed/min.at37°C).
ProteoChem"sImmobilizedPepsinispackagedasaslurryinbufferatpH4.5containing50%glycerol,100mMSodiumAcetate,0.05%NaN
3.
Pepsinproteasecleavesnon-specificallywithsomepreferencefortyrosinesandphenylalanine(aromaticresidues),althoughitgenerallydoesnotcleaveatalanine,glycineorvalineresidues.RecommendedconditionsarepH1-3,20°Cto37°C.