ImmobilizedChymotrypsinResin(ChymotrypsinAgarose)isusedtopreferentiallyhydrolyzeproteinsatTyrosine,Tryptophan,andPhenylalanineresidueswithminimalChymotrypsinautolysisbyproductsorotherproteasecontaminants.ImmobilizedChymotrypsinresinisprovidedina50%slurry,andissuppliedas2mLofactualresin(4mLofslurry).
ProteoChem"sImmobilizedChymotrypsingelismanufacturedfromsequencinggradeChymotrypsin,whichhasbeenTLCKtreatedtoremovetrypticactivity.ImmobilizedChymotrypsiniscovalentlyboundtoagarose,whichallowsforeasyremovaloftheenzymefromdigestedsample.RecommendedreactionconditionsarepH7.5to9.0at37ºC.